Product and Process Engineering - Delft University of Technology

Aswin Muralidharan

Aswin Muralidharan

Contact details

  • Aswin Muralidharan
  • Delft University of Technology
  • ChemE - PPE
  • Room E2.320
  • van der Maasweg 9, Delft 2629 HZ
  • The Netherlands
  • A.Muralidharan@tudelft.nl

Research interests and expertises

  • Transport Phenomena
  • Soft Condensed Matter Physics and Biophysics
  • Cell Mechanics
  • Particle Image Velocimetry

Social Media

Education

  • M.Sc.(Honours, Cum Laude), Chemical Engineering, Delft University of Technology, 2017
  • B.Tech., Chemical Engineering, National Institute of Technology, Calicut, India, 2014

Supervisors

Teaching Assistant

  • CH3131a, Applied Numerical Mathematics(M.Sc. Chemical Engineering, 2016-2017 Q1),
  • CH3053, Applied Transport Phenomena(M.Sc. Chemical Engineering, 2017-2018 Q2),

Research description

Application of strong electric fields of short duration render lipid membrane of cells to permeabilize enabling the transport of DNA and drugs possible. This phenomena, frequently referred to as electroporation, is a promising technology for non viral delivery of naked DNA molecules in cells or tissues. Although used in practice, current protocols for electroporation is far from optimal due to lack of knowledge in exact biophysical mechanisms involved.

At present, most of the biophysical studies on electropore formation and closure are performed on simple model cell systems like lipid bilayers and giant unilamellar vesicles. At the membrane level, the role of cytoskeleton in pore formation and closure is still lacking. This hinders creation of a good predictive model for flux of molecules into the cell.

At the cytosol, the journey of DNA from the membrane to the nucleus is an exciting transport phenomena problem. The exact biophysical details regarding active and passive transport of macromolecules inside the cytosol is still lacking. These information will help in developing better gene transfection technologies like nanochannel electroporation and disruption and field enhanced gene delivery.

. © Delft University of Technology - PPE group 2015