Product and Process Engineering - Delft University of Technology

Shaurya Sachdev

Shaurya Sachdev

Contact details

  • Shaurya Sachdev
  • Delft University of Technology
  • Dept. Chemical Engineering - PPE
  • Room E2.320
  • van der Maasweg, 9, 2629 HZ, Delft, The Netherlands
  • s.sachdev@tudelft.nl

Research interests and expertises

  • Transport Phenomena
  • Soft Matter and Biophysics
  • DNA Dynamics
  • Gene delivery

Education

  • MSc Chemical Engineering, Delft University of Technology, 2015
  • BE, Chemical Engineering, Birla Institute of Technology and Science, Pilani, Goa Campus, India (2013)

Supervisors

Research description

Application of electric field to cells transiently increases the permeability of the cell membrane allowing molecules including nucleotides carrying genetic material to enter into the cell. Therefore, electro-transfer of naked DNA molecules into cells and tissues (or electro-transfection) is being considered as a promising prospect for clinical gene delivery (electro-gene delivery). Till date the practical use of electro-gene delivery relies mostly on results of empirical studies and our lack of knowledge about the processes taking place at the cellular and the sub-cellular levels is preventing its widespread use. The exact biophysical mechanisms concerning the full itinerary of the DNA molecule, as it travels from outside of the cell to inside of the nucleus of the cell, are still unknown.

In my work, I will focus on events taking place at the membrane level and inside the cytoskeleton during electro-transfection. At the membrane level, I am interested in understanding how the DNA molecules translocate across the lipid bi-layer during the application of electric field. Inside the cytoskeleton I am interested in understanding how actin filaments and microtubules are involved in DNA internalization and their subsequent transfer to the nucleus.

Understanding these mechanisms both at the membrane level and inside the cytoskeleton will help us devise optimum electroporation protocols for electric field mediated gene delivery such as nano-channel electroporation (see 10.1038/nnano.2011.164).

    Electroporation ExperimentalSetup

Student projects

If you are interested in the topic, please contact me at s.sachdev@tudelft.nl, to discuss the possibilities.

. © Delft University of Technology - PPE group 2015