Product and Process Engineering - Delft University of Technology

Kartik Totlani

Kartik Totlani

Contact details

  • Kartik Arun Totlani
  • Delft University of Technology
  • Dept. Chemical Engineering - PPE & TP
  • Room F2.490
  • van der Maasweg 9, Delft, The Netherlands
  • k.a.totlani@tudelft.nl

Research interests and expertises

  • Droplet microfluidics
  • Biochemical reaction engineering in micro-droplets
  • Lab on a chip

Education

  • MSc, Chemical Engineering, Delft University of Technology, Netherlands, 2015
  • B.Tech, Chemical Engineering, Visvesvaraya National Institute of Technology, Nagpur, India, 2012

Supervisors

Research description

In current biotechnology practice screening of microbes is performed in batch mode, while most industrial reactors operate in fed-batch mode. It is well established that the performance of these microbes in nutrient rich environments (batch) differs from the performance in nutrient poor environments (fed-batch), explaining the limited success in microbe development. This project aims at development of a Lab on a chip platform for performing high throughput screening of microorganisms under industrially relevant conditions. In contrast to the conventional batch screening which leads to mutants failing to generate yields at an industrial scale, we want to use droplet microfluidics for screening the mutants in fed batch mode. By doing so, we wish to provide the same physiological environment to microbes during screening as it would have during the growth in the industrial fermentor. After developing such screening device we aspire to study the effect of different nutrient feed profiles on growth rate of microbes, nutrient consumption rates and yields of the desired metabolites. Eventually this technology would be validated by screening a well characterized yeast strain with it, thereby ensuring a quick adoption of this device by industry.

Student projects

If you are curious to know more about the topic and interested in carrying out your B.Sc./M.Sc. project, please contact me to discuss the possibilities

. © Delft University of Technology - PPE group 2015